The effect of pressure on fluorescence of tyrosine (Tyr) and Tyr in different concentration of Cu2+ was investigated. The results showed that Tyr fluorescence intensity was enhanced with increasing pressure in the absence of Cu2+, with the fluorescence intensity increasing by about 9% when the pressure reached 60 MPa. Furthermore, Tyr fluorescence quenched by Cu2+ and the quenching became stronger when the concentration of Cu2+ was higher. The effect of pressure on the fluorescence of Tyr was different under various Cu2+ concentrations. When Cu2+ concentration was lower, the fluorescence intensity increased relatively weakly (under 60 MPa and ［Copper ion］/［Tyr］=1, fluorescence intensity increased by 14.4%) and vice versa, the fluorescence intensity increased relatively strongly (under 60 MPa and ［Copper ion］/［Tyr］=40, fluorescence intensity increased by 38.4%).