Monitoring of wastewater discharge and diagnosis of pollution pattern are key problems in water quality prewarning. Because of differences of pollutants and their concentration, fluorescence spectrum is unique for each sample, named “fluorescence fingerprint”. The possibility to identify wastewater by fluorescence fingerprint parameters was investigated in the case of municipal wastewater from two plants. The major differences of municipal wastewater from two plants included that average fluorescence intensities of the highest peak (I280/340) and the secondarily highest peak (I225/340) of wastewater from A plant were 8308±1560 and 6350±1173; average I280/340 and average I225/340 of wastewater from B plant were 5929±400 and 4224±90; the average ratios of the highest peak intensity and secondarily highest peak intensity were 1.31 and 1.41, respectively. The fluorescence characteristics could be used to distinguish pollution pattern.