Wan LI, Haitao LONG, Weibing XU, Huali XUE, Wenqiang CAO, Zhaodi LI, and Lumei PU*
Fig. 1. Schematic diagram of experimental equipment
Fig. 2. Effects of single factor on germination index of Lycium barbarum: (a) time; (b) voltage; (c) electrolytic
Fig. 3. Contour and response surface diagram of the interaction of various factors on germination rate
Fig. 4. Contour and response surface diagram of the interaction of various factors on the vigor index
Fig. 5. Seed germination parameters for verification test
Fig. 6. Contact angle measurement images before and after treatment with CGDP: (a) control; (b) treatment; CA stands for contact angle
Fig. 7. Effects of CGDP treatment on seed surface contact angle
Fig. 8. Effects of contact glow discharge plasma treatment on seed coat morphology: (a) and (b) are SEM images for the untreated Lycium barbarum seed surface at magnifications of 1 000 × and 3 000 ×, respectively; (c) and (d) are corresponding images after 15 min plasma treatment
Fig. 9. Seedlings growth after transplanting for 30 d before (a, b, c) and after (d, e, f) CGDP treatment
Fig. 10. Effects of CGDP on chlorophyll content in Lycium barbarum leaves
Fig. 11. Schematic diagram of the effects of CGDP on seed germination and growth
水平Level | A / V | B / min | C |
---|
-1 | 530 | 10 | NaCl | 0 | 550 | 15 | Na2SO4 | 1 | 570 | 20 | KCl |
|
Table 1. Factors and level of Box-Behnken experiment
编号Number | A / V | B / min | C | R1 | R2 |
---|
1 | -1 | -1 | 0 | 91.333 3 | 346.713 | 2 | 1 | -1 | 0 | 90.000 0 | 320.114 | 3 | -1 | 1 | 0 | 87.777 8 | 306.376 | 4 | 1 | 1 | 0 | 91.111 1 | 343.953 | 5 | -1 | 0 | -1 | 87.777 8 | 256.360 | 6 | 1 | 0 | -1 | 85.555 6 | 249.853 | 7 | -1 | 0 | 1 | 86.666 7 | 284.298 | 8 | 1 | 0 | 1 | 88.888 9 | 265.792 | 9 | 0 | -1 | -1 | 88.888 9 | 246.460 | 10 | 0 | 1 | -1 | 82.222 2 | 191.746 | 11 | 0 | -1 | 1 | 84.444 4 | 179.377 | 12 | 0 | 1 | 1 | 87.912 0 | 214.098 | 13 | 0 | 0 | 0 | 94.444 4 | 605.356 | 14 | 0 | 0 | 0 | 94.444 4 | 595.438 | 15 | 0 | 0 | 0 | 93.333 3 | 580.363 | 16 | 0 | 0 | 0 | 94.444 4 | 620.587 | 17 | 0 | 0 | 0 | 93.333 3 | 579.246 |
|
Table 2. Box-Behnken design and results
响应值 Response value | 方差来源 Source | 平方和 Sum of squares | 自由度 Degrees of freedom | 均方 Mean square | F值 F value | p值 p value |
---|
Gr | Model | 218.01 | 9 | 24.22 | 78.07 | < 0.000 1 | | A-电压 A-Voltage | 0.50 | 1 | 0.50 | 1.61 | 0.244 9 | | B-时间 B-Time | 3.98 | 1 | 3.98 | 12.83 | 0.009 0 | | C-电解质 C-Electrolyte | 1.50 | 1 | 1.50 | 4.84 | 0.063 6 | | AB | 5.44 | 1 | 5.44 | 17.55 | 0.004 1 | | AC | 4.94 | 1 | 4.94 | 15.92 | 0.005 3 | | BC | 25.68 | 1 | 25.68 | 82.75 | < 0.000 1 | | A2 | 7.06 | 1 | 7.06 | 22.74 | 0.002 0 | | B2 | 29.57 | 1 | 29.57 | 95.29 | < 0.000 1 | | C2 | 126.59 | 1 | 126.59 | 408.00 | < 0.000 1 | | 残差Residual | 2.17 | 7 | 0.310 3 | - | - | | 失拟项Lack of fit | 0.690 5 | 3 | 0.230 2 | 0.6214 | 0.637 4 | | 纯误差Pure error | 1.48 | 4 | 0.370 4 | - | - | | 总离差Cor total | 220.18 | 16 | - | - | - | Vi | 模型Model | 4.150×105 | 9 | 4 6115.37 | 136.65 | < 0.000 1 | | A-电压 A-Voltage | 24.62 | 1 | 24.62 | 0.073 0 | 0.794 9 | | B-时间 B-Time | 166.45 | 1 | 166.45 | 0.493 2 | 0.505 2 | | C-电解质 C-Electrolyte | 0.091 0 | 1 | 0.091 0 | 0.000 3 | 0.987 4 | | AB | 1 029.66 | 1 | 1 029.66 | 3.05 | 0.124 2 | | AC | 35.99 | 1 | 35.99 | 0.106 6 | 0.753 5 | | BC | 1 999.65 | 1 | 1 999.65 | 5.93 | 0.045 1 | | A2 | 4 6754.69 | 1 | 46 754.69 | 138.55 | < 0.000 1 | | B2 | 1.099×105 | 1 | 1.099×105 | 325.55 | < 0.000 1 | | C2 | 2.165×105 | 1 | 2.165×105 | 641.48 | < 0.000 1 | | 残差 Residual | 2 362.27 | 7 | 337.47 | - | - | | 失拟项 Lack of fit | 1 144.89 | 3 | 381.63 | 1.25 | 0.401 8 | | 纯误差 Pure error | 1 217.39 | 4 | 304.35 | - | - | | 总离差 Cor total | 4.174×105 | 16 | - | - | - |
|
Table 3. Analysis of variance of regression model