• Journal of Radiation Research and Radiation Processing
  • Vol. 41, Issue 1, 010401 (2023)
Wan LI, Haitao LONG, Weibing XU, Huali XUE, Wenqiang CAO, Zhaodi LI, and Lumei PU*
Author Affiliations
  • College of Science, Gansu Agricultural University, Lanzhou 730000, China
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    DOI: 10.11889/j.1000-3436.2022-0103 Cite this Article
    Wan LI, Haitao LONG, Weibing XU, Huali XUE, Wenqiang CAO, Zhaodi LI, Lumei PU. Effects of contact glow discharge plasma on Lycium barbarum seed germination and seed treatment methodology[J]. Journal of Radiation Research and Radiation Processing, 2023, 41(1): 010401 Copy Citation Text show less
    Schematic diagram of experimental equipment
    Fig. 1. Schematic diagram of experimental equipment
    Effects of single factor on germination index of Lycium barbarum: (a) time; (b) voltage; (c) electrolytic
    Fig. 2. Effects of single factor on germination index of Lycium barbarum: (a) time; (b) voltage; (c) electrolytic
    Contour and response surface diagram of the interaction of various factors on germination rate
    Fig. 3. Contour and response surface diagram of the interaction of various factors on germination rate
    Contour and response surface diagram of the interaction of various factors on the vigor index
    Fig. 4. Contour and response surface diagram of the interaction of various factors on the vigor index
    Seed germination parameters for verification test
    Fig. 5. Seed germination parameters for verification test
    Contact angle measurement images before and after treatment with CGDP: (a) control; (b) treatment; CA stands for contact angle
    Fig. 6. Contact angle measurement images before and after treatment with CGDP: (a) control; (b) treatment; CA stands for contact angle
    Effects of CGDP treatment on seed surface contact angle
    Fig. 7. Effects of CGDP treatment on seed surface contact angle
    Effects of contact glow discharge plasma treatment on seed coat morphology: (a) and (b) are SEM images for the untreated Lycium barbarum seed surface at magnifications of 1 000 × and 3 000 ×, respectively; (c) and (d) are corresponding images after 15 min plasma treatment
    Fig. 8. Effects of contact glow discharge plasma treatment on seed coat morphology: (a) and (b) are SEM images for the untreated Lycium barbarum seed surface at magnifications of 1 000 × and 3 000 ×, respectively; (c) and (d) are corresponding images after 15 min plasma treatment
    Seedlings growth after transplanting for 30 d before (a, b, c) and after (d, e, f) CGDP treatment
    Fig. 9. Seedlings growth after transplanting for 30 d before (a, b, c) and after (d, e, f) CGDP treatment
    Effects of CGDP on chlorophyll content in Lycium barbarum leaves
    Fig. 10. Effects of CGDP on chlorophyll content in Lycium barbarum leaves
    Schematic diagram of the effects of CGDP on seed germination and growth
    Fig. 11. Schematic diagram of the effects of CGDP on seed germination and growth
    水平LevelA / VB / minC
    -153010NaCl
    055015Na2SO4
    157020KCl
    Table 1. Factors and level of Box-Behnken experiment
    编号NumberA / VB / minCR1R2
    1-1-1091.333 3346.713
    21-1090.000 0320.114
    3-11087.777 8306.376
    411091.111 1343.953
    5-10-187.777 8256.360
    610-185.555 6249.853
    7-10186.666 7284.298
    810188.888 9265.792
    90-1-188.888 9246.460
    1001-182.222 2191.746
    110-1184.444 4179.377
    1201187.912 0214.098
    1300094.444 4605.356
    1400094.444 4595.438
    1500093.333 3580.363
    1600094.444 4620.587
    1700093.333 3579.246
    Table 2. Box-Behnken design and results

    响应值

    Response value

    方差来源

    Source

    平方和

    Sum of squares

    自由度

    Degrees of freedom

    均方

    Mean square

    F

    F value

    p

    p value

    GrModel218.01924.2278.07< 0.000 1
    A-电压 A-Voltage0.5010.501.610.244 9
    B-时间 B-Time3.9813.9812.830.009 0
    C-电解质 C-Electrolyte1.5011.504.840.063 6
    AB5.4415.4417.550.004 1
    AC4.9414.9415.920.005 3
    BC25.68125.6882.75< 0.000 1
    A27.0617.0622.740.002 0
    B229.57129.5795.29< 0.000 1
    C2126.591126.59408.00< 0.000 1
    残差Residual2.1770.310 3--
    失拟项Lack of fit0.690 530.230 20.62140.637 4
    纯误差Pure error1.4840.370 4--
    总离差Cor total220.1816---
    Vi模型Model4.150×10594 6115.37136.65< 0.000 1
    A-电压 A-Voltage24.62124.620.073 00.794 9
    B-时间 B-Time166.451166.450.493 20.505 2
    C-电解质 C-Electrolyte0.091 010.091 00.000 30.987 4
    AB1 029.6611 029.663.050.124 2
    AC35.99135.990.106 60.753 5
    BC1 999.6511 999.655.930.045 1
    A24 6754.69146 754.69138.55< 0.000 1
    B21.099×10511.099×105325.55< 0.000 1
    C22.165×10512.165×105641.48< 0.000 1
    残差 Residual2 362.277337.47--
    失拟项 Lack of fit1 144.893381.631.250.401 8
    纯误差 Pure error1 217.394304.35--
    总离差 Cor total4.174×10516---
    Table 3. Analysis of variance of regression model
    Wan LI, Haitao LONG, Weibing XU, Huali XUE, Wenqiang CAO, Zhaodi LI, Lumei PU. Effects of contact glow discharge plasma on Lycium barbarum seed germination and seed treatment methodology[J]. Journal of Radiation Research and Radiation Processing, 2023, 41(1): 010401
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