Non-uniform illumination of high-throughput microfluidic qPCR

Huadong WANG; Shumi ZHAO; Ling ZHU; Zhigang LI; An WANG; Yong LIU

doi:10.3969/j.issn.1007-5461. 2015.05.016

[1] Zhu Ling, Zhu Cancan, Deng Guoqing, et al. Rapid identification of H5 avian influenza virus in chicken throat swab specimens using microfluidic real-time RT-PCR［J］. Analytical Methods, 2014, (8): 2628-2632.

[2] Corman V M, Eickmann M, Landt O, et al. Specific detection by real-time reverse-transcription PCR assays of a novel avian influenza A(H7N9) strain associated with human spillover infections in China［J］. Euro. Surveill, 2013, 18(16): 10-16.

[4] Shen F, Du W, Kreutz J E, et al. Digital PCR on a SlipChip［J］. Lab Chip, 2010, 10(20): 2666-2672.

[5] Hatch A C, Fisher J S, Tovar A R, et al. 1-Million droplet array with wide-field fluorescence imaging for digital PCR［J］. Lab Chip, 2011, 11(22): 3838-3845.

[7] Ding Yi, Liu Xu, Zheng Zhenrong, et al. Freeform LED lens for uniform illumination［J］. Opt. Expr., 2008, 1 (17): 12958-12966.

[8] Su Zhouping, Xue Donglin, Ji Zhicheng. Designing LED array for uniform illumination distribution by simulated annealing algorithm［J］. Opt. Expr., 2012, 20(106): A843-A855.

[10] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method［J］. Methods, 2001, 25(4): 402-408.

[11] Karlen Y, McNair A, Perseguers S, et al. Statistical significance of quantitative PCR［J］. BMC Bioinformatics, 2007, 8(1): 131-146.

[12] Schmittgen T D, Livak K J. Analyzing real-time PCR data by the comparative CT method［J］. Nature Protocols, 2008, 3(6): 1101-1108.

[13] Larionov A, Krause A, Miller W. A standard curve based method for relative real time PCR data processing［J］. BMC Bioinformatics, 2005, (1): 62-77.