Shenghui Shi, Decao Wu, Xin Wang, Qinglin Nie, Zhijiang Liu, Binbin Luo, Enhua Liu, Peng Liu, Mingfu Zhao. An Immunosensor Based on the Graphene-Oxide-Encapsulated Au-Nanoshell-Coated Long-Period Fiber Grating[J]. Acta Optica Sinica, 2020, 40(18): 1806001
- Acta Optica Sinica
- Vol. 40, Issue 18, 1806001 (2020)
Fig. 1. Mechanism for encapsulation of graphene oxide with AuNS
Fig. 2. Fabrication of the EGO-AuNS-LPFG immunosensor. (a) Surface pre-treatment; (b) hydroxylation; (c) silylation; (d) modification with EGO-AuNS; (e) activation of carboxyl group; (f) surface modification with AIV-MAbs and sealing of redundant binding sites; (g) detection for AIV antigens
Fig. 3. Schematic diagram of the experimental system
Fig. 4. FESEM graphs of the EGO-AuNS-LPFG images. (a) 5000×; (b) 10000×; (c) 50000×
Fig. 5. Energy spectrum of the EGO-AuNS-LPFG
Fig. 6. Transmission spectrum of the EGO-AuNS-LPFG
Fig. 7. Transmission spectra of the bare LPFG and the EGO-AuNS coated LPFG immersed in different sucrose concentrations. (a) Bare LPFG; (b) EGO-AuNS coated LPFG
Fig. 8. Resonant wavelength of the bare LPFG and EGO-AuNS coated LPFG against RI of different sucrose solutions
Fig. 9. Spectral evolution and the corresponding wavelength shift of the immunosensor for AIV detection against time. (a) Spectral evolution;(b)corresponding wavelength shift
Fig. 10. Wavelength shift of the GO-AuNS-LPFG immunosensor for different AIV concentration levels (Inset corresponds to the linear fitting area)
Fig. 11. Wavelength shift for specificity and clinical assays of the EGO-AuNS-LPFG immunosensor
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Table 1. Corresponding percentage content for the surface of the EGO-AuNS-LPFG%
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