Using Gutzwiller's periodic orbit theory, we study the quantum level density of a spherical billiard in the presence of a magnetic flux line added at its center, especially discuss the influence of the magnetic flux strength on the quantum level density. The Fourier transformed quantum level density of this system has allowed direct comparison between peaks in the level density and the length of the periodic orbits. For particular magnetic flux strength, the amplitude of the peaks in the level density decreased and some of the peaks disappeared. This result suggests that Aharonov-Bohm effect manifests itself through the cancellation of periodic orbits. This phenomenon will provide a new experimental testing ground for exploring Aharonov-Bohm effect.