To calibrate the intrinsic and extrinsic parameters of cameras in motion parameters photoelectric measurement system,a novel approach based on improved evolutionary strategy and surveying a rigid one-dimensional object carrying three markers on its middle and extremities moved inside the working volume,which does not require other complex calibration equipments,was proposed in this paper.Firstly,it is assumed that the principal point is located at a certain location around the image plane center.Then the focal length of cameras,the rotation matrix,translation vector and the scale factor are obtained.Finally,the estimation of intrinsic and extrinsic parameters of cameras is formulated as a nonlinear minimization problem of a cost function.The simulated annealing evolutionary strategy algorithm was improved by introducing the self-improved factor and the Euclidean distance between individual.Consequently the global convergence performance of the search algorithm of this method was mended and accelerated.By introducing the evolutionary strategy,the new method can find out the intrinsic and extrinsic parameters all together,and need not constrain the movement of the one-dimensional object in comparison with traditional methods.