The normal Raman (NR) and surface enhanced Raman spectroscopy (SERS) for Alternaria alternate, Colletotrichum musae, and fusarium suspensions were measured by a portable Raman spectrometer (785 nm). The result indicates that the silver colloid prepared by microwave method shows high enhancement of Raman scattering for the three kinds of pathogens. Comparing SERS spectrums of the three kinds of pathogens, there are some similarities. In these SERS spectrums, the peaks in the range of 500~1 000 cm-1 are very weak. Additionally, the peaks in the range of 1 000~1 600 cm-1 are strong. Meanwhile, the peak at 481 cm-1 is the strongest. Although these spectrums are similar, there are some differences such as the distribution and shape of peaks. Therefore, the three kinds of pathogens can be discriminated quickly.