The golden buckwheat Fagopyrum dibotrys produced in Yunnan has a unique anti-cancer effects.It is a main raw material of “Wei Mai ning” capsules which is the national second-class anti-cancer drug. The present paper used(5∶1) mixed acid as digestive juice to process the sample, and determine the twelve elements including K, Ca, Cu, Na, Mg, Mn, Fe, Zn, Pb, Cr, Cd and Co in the Fagopyrum dibotrys by inductively coupled plasma atomic emission spectrometry(ICP-AES). The detection limits of this method were 0.017～0.084 μg·mL-1, the RSDs (n=8) were all 0.09%～1.87%, and the addition standard recoveries(ASR)(n=8) were 98.2%～107.4% for all elements. The research results showed that there is rich K(1 477.3 μg·g-1） in the Fagopyrum dibotrys, there are not harmful elements Cd and Pb, and this result is mainly related to the geochemistry background where the sample lived. The contents of seven remaining kinds of elements ranked as Na(826.1）>Ca(765.2>Mg(493.4）>Zn(112.7）>Fe(56.5）>Cu(11.4）>Mn(4.49 μg·g-1）. This result provides some theoretical basis for the study of internal relations between trace elements in Fagopyrum dibotrys and efficacy. It’ s also useful for better development and utilization of the resource.