Using three-dimensional fluorescence technology,we studied fluorescent characteristics of two polluted rivers by a surface flow+vertical flow combined constructed wetlands of dissolving organic matter.The results showed that (1) the main sources of water-soluble humic organic matter in constructed wetland was biological metabolic input instead of terrigenous input;　(2) in the later section of the surface flow constructed wetland,part of proteinoid substance changed into fulvic acid-like substance,which showed that the composition of dissolved organic matter and material structure tended to be stable after surface flow combined constructed wetland treatment;　(3) it was of great significance that surface flow constructed wetland in structure transformation of water soluble organic matter,which could significantly improve the stability of water soluble organic matter.Surface flow+vertical flow combined constructed wetland process of dissolved organic matter had a good removal effect.