The impact of microwave sweeper power fluctuation in the BOTDA system has been theoretically analyzed and experimentally tested. And a novel method comparing real-time acquisition of probe wave power with a new algorithm to realize probe wave power normalization for eliminating this impact was proposed. The principle of the proposed method was described theoretically. And the contrast test between our new method and conventional one was carried out. The experiment results indicated that the temperature accuracy was effectively improved from ±5 ℃ to ±2 ℃.