A method for simultaneously analysis of inorganic elements in rare and endangered plant spinulose tree fern was established. Alsophila Spinulosa samples were pretreated by using the hermetic microwave digestion. Six elements Na, Mg, Al, P, K and Ca were determined by using the inductively coupled plasma optical emission spectrometry (ICP-OES) and ten elements V, Cr, Mn, Fe, Ni, Cu, Zn, Cd, Hg and Pb were determined by using inductively coupled plasma mass spectrometry (ICP-MS). The working conditions were optimized as well. In order to keep the stability of the experiment, low concentration of HCl was added into the sample during testing. Multicomponent Spectral Fitting (MSF) and dynamic reaction cell (DRC) were used to correct the spectral interference and mass interference. Under optimized conditions, the standard linear relationships were satisfactory and the detection limit ranged from 1.87 ng·L-1～12.31 μg·L-1. Finally, the standard reference material NIST SRM 1 547 (peach leaves) was used to verify the proposed method. The obtained results were consistent with the reference method with the sameaccuracy and precision. Two Alsophila Spinulosa samples analysis results showed that Alsophila Spinulosa have higher concentrations of Na, Mg, K, Ca, Al, and P, and concentration of K is the highest. These results indicated that Alsophila Spinulosa have strong capability in K-enrichment. Alsophila Spinulosa have low concentrations of V, Cr, Mn, Fe, Cu, and Zn. Concentrations of Ni, Cd, Hg, and Pb are at very low trace levels, which indicated that the wild living environment is very healthy. This method has features of fast analyzing, high accuracy and convenience in operation, which can provide scientific basis for Alsophila Spinulosa inorganic elements contents measure.