The nonlinear acousto-optic tunable filter (AOTF) based on TeO₂ is a new device which can convert the ultrasonic and optical wave. It has small volume, low weight, large aperture and incident aperture, rapid coordination in a wide spectral range, and good diffraction efficiency. The AOTF based on TeO₂ is used for hyperspectral imaging experiments. The conversion between diffraction of light central wavelength and ultrasonic frequency in the experiment is very close to the theoretical result. A hyperspectral microscopic imaging system, combined with an inverted optical microscope, which can exhibit histologic section of human parietal cells under different light central wavelength and different ultrasonic frequency on AOTF is designed. The experimental results show that the image quality is very good. By comparing images under different ultrasonic wave frequency and light centeral wavelength, more details and difference of cells can be found. A method to acquire hyperspectral images of cells under different light central wavelength is provided, the method is easy to operate and the results are easy to analyze.