The spectral properties of odd-parity highly excited states of europium Eu atom have been investigated with the technique of two-color three-photon resonant ionization spectroscopy in the energy region of 42896-44854 cm-1. The spectral information of 93 highly excited states of europium atom has been reported in this energy region. On the one hand,three different excitation pathways are used,not only the energy values of these states have been measured,but also the relative strengths of their photoionization signals are given. On the other hand,according to the selection rules of the electric dipole transition,the total angular momentum quantum numbers J of these states have been uniquely determined by analyzing and comparing the spectral data obtained in the experiment. Comparisons with the published literatures,25 new odd-parity highly excited states of europium atom have been discovered,which lie in the region of the present investigation,except for confirming some of the states reported in the literatures. In addition,the spectral information of several highly excited states from the literatures has been amended,and the J values of these states also have been assigned uniquely.