• Spectroscopy and Spectral Analysis
  • Vol. 41, Issue 2, 379 (2021)
Shao-hua LI1、1、*, Hong-xia ZHAO1、1, Chen WEN1、1, Zhi-qun DING1、1, Jing-rui WANG1、1, and Pei-hong CHENG1、1
Author Affiliations
  • 1[in Chinese]
  • 11. School of Electronics and Information Engineering, Ningbo University of Technology, Ningbo 315211, China
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    DOI: 10.3964/j.issn.1000-0593(2021)02-0379-09 Cite this Article
    Shao-hua LI, Hong-xia ZHAO, Chen WEN, Zhi-qun DING, Jing-rui WANG, Pei-hong CHENG. Application and Development of Photothermal Based Microscopy in Single Particle Detection[J]. Spectroscopy and Spectral Analysis, 2021, 41(2): 379 Copy Citation Text show less
    Schematics of measurement principle of single-particle photothermal effect
    Fig. 1. Schematics of measurement principle of single-particle photothermal effect
    Schematics of the experiment set-up
    Fig. 2. Schematics of the experiment set-up
    (a) Schematic of photothermal lens phenomena; (b) Schematic illustration of the photothermal measurement of a nanoparticle in liquid microspace
    Fig. 3. (a) Schematic of photothermal lens phenomena; (b) Schematic illustration of the photothermal measurement of a nanoparticle in liquid microspace
    Monitored signals for the trace turbid solution of 80 nm polystyrene particles. The expectation values were (a) 8.7×10-4 , (b) 1.8×10-4, and (c) 3.6×10-5, (d) Ultrapure water (blank)
    Fig. 4. Monitored signals for the trace turbid solution of 80 nm polystyrene particles. The expectation values were (a) 8.7×10-4 , (b) 1.8×10-4, and (c) 3.6×10-5, (d) Ultrapure water (blank)
    (a) Schematics of the experiment set-up; (b) photothermal images of 5 nm gold nanoparticles in a few tens of nanometers thick polyvinyl alcohold film on a glass substrate
    Fig. 5. (a) Schematics of the experiment set-up; (b) photothermal images of 5 nm gold nanoparticles in a few tens of nanometers thick polyvinyl alcohold film on a glass substrate
    (a) Experiment Schematics of photothermal heterodyne imaging method; (b) photothermal imaging of isolated 5 nm Au nanoparticles; (c) photothermal image of isolated 1.4 nm Au nanoparticle
    Fig. 6. (a) Experiment Schematics of photothermal heterodyne imaging method; (b) photothermal imaging of isolated 5 nm Au nanoparticles; (c) photothermal image of isolated 1.4 nm Au nanoparticle
    Photothermal images of 28 nm gold nanoparticles recorded in different media(b): Water; (c): Silicone oil; (d): 5CB at 23 ℃; (e): 5CB at 31 ℃
    Fig. 7. Photothermal images of 28 nm gold nanoparticles recorded in different media
    (b): Water; (c): Silicone oil; (d): 5CB at 23 ℃; (e): 5CB at 31 ℃
    (a) Photothermal correlation curves obtained from 10 nm biotin monofunctionalized nanoparticles(left) and upon mixture with streptavidin. Solid lines represent theoretical fits using eq 2; (b) Photothermal curves from bare and CALNN-coated gold nanoparicles. CALNN is a 5 amino-acid long peptide
    Fig. 8. (a) Photothermal correlation curves obtained from 10 nm biotin monofunctionalized nanoparticles(left) and upon mixture with streptavidin. Solid lines represent theoretical fits using eq 2; (b) Photothermal curves from bare and CALNN-coated gold nanoparicles. CALNN is a 5 amino-acid long peptide
    Experimental setup of photothermal heterodyne holography
    Fig. 9. Experimental setup of photothermal heterodyne holography
    Shao-hua LI, Hong-xia ZHAO, Chen WEN, Zhi-qun DING, Jing-rui WANG, Pei-hong CHENG. Application and Development of Photothermal Based Microscopy in Single Particle Detection[J]. Spectroscopy and Spectral Analysis, 2021, 41(2): 379
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