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    Journals >Journal of Inorganic Materials >Volume 35 >Issue 2 >Page 187 > Article
    • Journal of Inorganic Materials
    • Vol. 35, Issue 2, 187 (2020)
    Construction of Polyethyleneimine-modified Dual-mesoporous Silica Gene Carrier
    Mei-Rong FANG1, Li-Mei QIN2, Xiao-Bo JIA2, Yong-Sheng LI2, De-Chao NIU2、*, and Ze-Lan HU1、*
    Author Affiliations
  • 1Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
  • 2School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China
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    DOI: 10.15541/jim20190099 Cite this Article
    Mei-Rong FANG, Li-Mei QIN, Xiao-Bo JIA, Yong-Sheng LI, De-Chao NIU, Ze-Lan HU. Construction of Polyethyleneimine-modified Dual-mesoporous Silica Gene Carrier[J]. Journal of Inorganic Materials, 2020, 35(2): 187 Copy Citation Text show less
    TEM images of EDMSNs-PEI (a) and EDMSNs- NH2 (b)
    1. TEM images of EDMSNs-PEI (a) and EDMSNs- NH2 (b)
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    Zeta potentials (a) and particle size distributions (b) of EDMSNs, EDMSNs-PEI and EDMSNs-NH2
    2. Zeta potentials (a) and particle size distributions (b) of EDMSNs, EDMSNs-PEI and EDMSNs-NH2
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    N2 adsorption-desorption isotherms and the corresponding DFT pore size distributions of EDMSNs-PEI (a-b) and EDMSNs-NH2 (c-d)
    3. N2 adsorption-desorption isotherms and the corresponding DFT pore size distributions of EDMSNs-PEI (a-b) and EDMSNs-NH2 (c-d)
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    Electrophoresis diagram of EDMSNs-PEI/pDNA and EDMSNs-NH2/pDNA nanoparticles at different weight ratios (a), DNaseI digestion electrophoresis diagram (b) and zeta potentials (c) of EDMSNs-PEI/pDNA with M indicating DNA marker and C indicating naked pDNA
    4. Electrophoresis diagram of EDMSNs-PEI/pDNA and EDMSNs-NH2/pDNA nanoparticles at different weight ratios (a), DNaseI digestion electrophoresis diagram (b) and zeta potentials (c) of EDMSNs-PEI/pDNA with M indicating DNA marker and C indicating naked pDNA
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    Cytotoxicity assay of PEI 25kD, LIP2K and EDMSNs-PEI in 293T (a) and u87(b). * p<0.01, EDMSNs-PEI
    5. Cytotoxicity assay of PEI 25kD, LIP2K and EDMSNs-PEI in 293T (a) and u87(b). * p<0.01, EDMSNs-PEI
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    Transfection efficacy of EDMSNs-PEI/pDNA nanoparticle in 293T cell lines.
    6. Transfection efficacy of EDMSNs-PEI/pDNA nanoparticle in 293T cell lines.
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    Abstract
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    Mei-Rong FANG, Li-Mei QIN, Xiao-Bo JIA, Yong-Sheng LI, De-Chao NIU, Ze-Lan HU. Construction of Polyethyleneimine-modified Dual-mesoporous Silica Gene Carrier[J]. Journal of Inorganic Materials, 2020, 35(2): 187
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