Based on the objective lens and voice coil motor of the current DVD pickup head,according to photobleaching three-dimensional (3D) optical information storage's theory of two-photon absorption,the Ti∶sapphire femtosecond pulse laser was used as written optical beam and reading optical beam of two-photon three-dimensional optical storage voice coil motor was carried through multiplayer choice, and the storage and readout experimental study of binary format code with two-photon photobleaching in the new photobleaching material consisting of stilbene derivant was conducted. Three layers optical data storage was realized,the two adjacent bits distance in each layer was 4 μm and the two adjacent layers interval was 15 μm. The signal intensity of the written information was readout and identified with Matlab software,and the identified result matchesd with the written binary format code。The experiment proved that proceeding two-photon three-dimensional optical data storage with DVD pickup head was feasible, which indicated that two-photon photobleaching technology combined with the current CD/DVD and provided a base for realization of high density and super high density optical data storage.