A DOA (Direction of Arrival) estimation method based on 2D-Unitary ESPRIT algorithm with hexangular star array is proposed.The multiple translation invariance of subarrays from hexangular star array is used to form combinations of each two set of subarrays.2D-Unitary ESPRIT algorithm is used to respectively estimate every combination， obtaining the candidate set of DOA estimation;then the power spectral function of 2D-MUSIC algorithm is adopted for optimization estimation of DOA in the candidate set， obtaining the optimized estimated value of DOA.Simulation experiment is conducted.The proposed method avoids the huge computational cost caused by spectral peak search of 2D-MUSIC algorithm， and DOA estimation error is reduced by applying combinations of multiple translation invariance subarrays.The simulation shows that:Compared with the traditional 2D-Unitary ESPRIT algorithm， the proposed method can reduce the mean square root error of estimated value and improve the spatial resolution.