IR and UV-Vis was employed to analyzed the spectrum effect of hydrogen bonding on the partially hydrolyzed polyacrylamide polymer(HPAM). The study reveals that, the characteristic absorption peak of free amino group moves to the low frequency due to the formation of intramolecular hydrogen bonding between amide group and carboxyl group. In the water solution, intramolecular hydrogen bonding is the main factor that shifted maximal absorption towards long wavelength. Intramolecular hydrogen bonding and intermolecular hydrogen bonds exist at the same time in the water solution contains intermediate sodium and calcium ions. While in the high concentration solution, the main form between amide group and carboxyl group is intermolecular hydrogen bonding. The effect of hydrogen bonding on the spectrum of HPAM demonstrates different extent in various systems. In the water solution, the maximum absorption wavelength red shifts 8 nm. In the system contains sodium ions, this shift is 4 nm. And this shift is only 2 nm in the solution contains both sodium ions and calcium ions.