We reported a single-frequency fiber laser at 915 nm based on the neodymium-doped yttrium aluminum garnet (Nd∶YAG) crystal-derived fiber (NYDF). Using Nd∶YAG crystal with doped atomic number fraction of 2.5% as the core material and a high-purity silica tube as the cladding material, we fabricated the NYDF by the molten-core method. Its transmission loss was 8 dB/m and its gain coefficient at 915 nm was 1.16 dB/cm. A stable single-frequency fiber laser at 915 nm based on the NYDF was developed, with a signal-to-noise ratio of above 50 dB. The experimental results show that the NYDF is a promising candidate material for single-frequency lasers at 890 nm--920 nm waveband.