Developing a contact probe for rodent fundus imaging in a confocal scanning laser ophthalmoscope

Xiaoyun Jiang; Yichen Ding; Wenyao Wang; Zhiyu Huang; Zhiru Wang; Elie de Lestrange Anginieur; Yue Yu; Jun Li; Mingliang Pu; Qiushi Ren; Changhui Li

doi:10.3788/COL201614.031701

Journals >Chinese Optics Letters >Volume 14 >Issue 3 >Page 031701 > Article

Chinese Optics Letters
Vol. 14, Issue 3, 031701 (2016)

Developing a contact probe for rodent fundus imaging in a confocal scanning laser ophthalmoscope

Xiaoyun Jiang¹, Yichen Ding¹, Wenyao Wang², Zhiyu Huang¹, Zhiru Wang^3、4, Elie de Lestrange Anginieur¹, Yue Yu¹, Jun Li³, Mingliang Pu², Qiushi Ren¹, and Changhui Li^1、*

Author Affiliations

¹Department of Biomedical Engineering, Peking University, Beijing 100871, China

²Department of Anatomy, School of Basic Medical Sciences, Peking University, Beijing 100191, China

³Laboratory Animal Center, Peking University, Beijing 100871, China

⁴School of Public Health, Jilin University, Changchun 130012, China

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DOI: 10.3788/COL201614.031701 Cite this Article Set citation alerts

Xiaoyun Jiang, Yichen Ding, Wenyao Wang, Zhiyu Huang, Zhiru Wang, Elie de Lestrange Anginieur, Yue Yu, Jun Li, Mingliang Pu, Qiushi Ren, Changhui Li. Developing a contact probe for rodent fundus imaging in a confocal scanning laser ophthalmoscope[J]. Chinese Optics Letters, 2016, 14(3): 031701 Copy Citation Text

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Schematic design of the probe. (a) The structure of the contact probe for rats; (b) the surface of the soft gel can slightly change shape to adapt to different rodent eyes.

Fig. 1. Schematic design of the probe. (a) The structure of the contact probe for rats; (b) the surface of the soft gel can slightly change shape to adapt to different rodent eyes.

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Fig. 2. Making the concave surface with a steel ball.

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Fig. 3. Comparison between the flexible contact probe (a, c) and plano–concave method (b, d) in the spot diagram and PSF.

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Fig. 4. Layout of the CSLO system for rodents, and a photo of the contact probe with the subject. L: lens, DM: dichroic mirror, PBS: polarizing beam splitter, QWP: quarter wave plate.

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Fig. 5. In vivo retinal imaging. Comparison of the reflectance image by (a) the flexible contact probe and (b) the plano–concave lens for a rat; (c) autofluorescence imaging and (d) FA for the same rat; (e) reflectance and (d) fluorescence imaging for amouse. Scale bar: 100 μm.

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	Average axial length (mm)	Total power (D)	Average refractive error (D)	NA
Human	$\sim 23.5 - 24$	$\sim 60$	$\sim 0$ to $+ 1$	$\sim 0.20$
Rat	$\sim 6.1$	$\sim 300$	$+ 5$ to $+ 15$	$\sim 0.43$
Mouse	$\sim 3.3$	$\sim 560$	$+ 7$ to $+ 15$	$\sim 0.49$