Based on the propagation model of optical pulse in doped fiber, the split-step Fourier method is used to numerically study the generation and the transmission of Peregrine soliton in doped fiber. Based on the Peregrine soliton solution, the generation and the transmission of Peregrine soliton in doped fiber are discussed. The peak pulses of Peregrine soliton are extracted, the background waves are eliminated, and the transmission characteristics of high peak pulses are discussed. The results show that, when it is transmitted in doped fiber, the Peregrine soliton generates a high peak single pulse that is localized temporally and spatially, and then the high peak pulse splits rapidly to produce multiple sub-pulses. The saturated energy and the pulse peak intensity increase with the increasing small signal gain, the pulse width declines with the increasing gain, and the spatial separations of the excited sub-pulses are gradually reduced. When the background wave of the high peak pulse is eliminated, the pulse can be transmitted stably in doped fiber, the pulse width presents breath-type periodic change with periodic oscillation of the pulse intensity, and the average value of the pulse intensity is gradually increasing.