Changes of Tumor Cell Components Induced by Pain-Related Neuropeptides SP Investigated by Synchrotron Radiation Infrared Microscopic Imaging Technology

Chao YANG; Zhen ZHU; Jing LI; Yun-heng SUN; Xue-yan OUYANG; Jia-wei WANG; Qian HUANG; Gang DING; Yao-sheng WANG; Feng JIANG

doi:10.3964/j.issn.1000-0593(2020)11-3579-06

Journals >Spectroscopy and Spectral Analysis >Volume 40 >Issue 11 >Page 3579 > Article

Spectroscopy and Spectral Analysis
Vol. 40, Issue 11, 3579 (2020)

Changes of Tumor Cell Components Induced by Pain-Related Neuropeptides SP Investigated by Synchrotron Radiation Infrared Microscopic Imaging Technology

Chao YANG^1、1, Zhen ZHU^1、1、*, Jing LI^1、1, Yun-heng SUN^1、1, Xue-yan OUYANG^1、1, Jia-wei WANG^1、1, Qian HUANG^1、1, Gang DING^1、1, Yao-sheng WANG^1、1, and Feng JIANG^1、1

Author Affiliations

¹[in Chinese]

¹1. Translational Institute for Cancer Pain, Xinhua Hospital Chongming Branch, Shanghai 202150, China

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DOI: 10.3964/j.issn.1000-0593(2020)11-3579-06 Cite this Article

Chao YANG, Zhen ZHU, Jing LI, Yun-heng SUN, Xue-yan OUYANG, Jia-wei WANG, Qian HUANG, Gang DING, Yao-sheng WANG, Feng JIANG. Changes of Tumor Cell Components Induced by Pain-Related Neuropeptides SP Investigated by Synchrotron Radiation Infrared Microscopic Imaging Technology[J]. Spectroscopy and Spectral Analysis, 2020, 40(11): 3579 Copy Citation Text

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Fig. 1. FTIR spectra of four cell lines

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Range /cm^-1	Peak position/cm^-1	Possible resolve
990~1 040	1 029	C—O—, C—O—H
1 040~1 070	1 054	C—O stretching
1 070~1 090	1 083	P=O stretching
1 150~1 190	1 165	C—O stretching
1 230~1 250	1 240	P=O stretching
1 430~1 470	1 455	—CH₂ deformation
1 500~1 600	1 551	Amide Ⅱ
1 600~1 700	1 655	Amide Ⅰ
1 700~1 800	1 747	C=O stretching
2 910~2 950	2 930	C—H stretching
2 950~2 990	2 970
3 300~3 500	3 383	O—H, N—H stretching

Table 1. The bands frequencies assigned for the IR spectra

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		MDA-MB-231(Mean±SD)		P-value	MCF-7(Mean±SD)		P-value
		Control(n=12)	SP(n=12)	P-value	Control(n=12)	SP(n=12)	P-value
Range /cm^-1	1 070~1 090	1 076.69±6.56	1 077.241±4.572	0.854	1 079.764±2.19	1 078.05±2.025	0.082
	1 230~1 250	1 235.383±2.916	1 234.832±6.171	0.848	1 237.656±4.917	1 237.656±7.766	0.51
	1 380~1 420	1 396.005±2.062	1 397.934±4.648	0.134	1 396.763±3.38	1 395.316±4.25	0.272
	1 430~1 470	1 452.760±4.944	1 453.587±5.068	0.732	1 459.441±7.968	1 460.405±4.696	0.401
	1 500~1 600	1 548.086±0.941	1 538.719±1.712	<0.001	1 547.432±3.082	1 538.03±4.25	0.002
	1 600~1 700	1 651.678±4.705	1 639.555±3.419	0.001	1 647.235±4.426	1 636.869±3.391	<0.001
	2 910~2 950	2 925.311±4.121	2 925.247±4.181	0.9	2 925.632±1.897	2 924.347±0.996	0.209
	2 950~2 990	2 959.244±1.517	2 959.244±2.187	0.999	2 958.418±1.604	2 958.9±0.893	0.241
	3 300~3 500	3 295.366±1.834	3 295.488±2.396	0.929	3 292.542±1.996	3 292.059±5.646	0.417

Table 2. Spectral peak analysis of breast cancer cell lines

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		MDA-MB-231(Mean±SD)		P-value	MCF-7(Mean±SD)		P-value
		Control(n=12)	SP(n=12)	P-value	Control(n=12)	SP(n=12)	P-value
Range /cm^-1	1 070~1 090	0.52±0.129	0.581±0.182	0.415	0.33±0.07	0.32±0.087	0.838
	1 230~1 250	0.382±0.088	0.324±0.166	0.705	0.257±0.086	0.204±0.068	0.262
	1 380~1 420	0.381±0.084	0.394±0.121	0.516	0.278±0.072	0.299±0.074	0.632
	1 430~1 470	0.377±0.082	0.416±0.11	0.255	0.282±0.061	0.328±0.074	0.284
	1 500~1 600	0.663±0.073	0.691±0.095	0.29	0.588±0.065	0.583±0.095	0.917
	1 600~1 700	0.965±0.057	0.886±0.101	0.274	0.896±0.076	0.739±0.134	0.058
	2 910~2 950	0.417±0.057	0.406±0.056	0.788	0.54±0.045	0.511±0.02	0.158
	2 950~2 990	0.386±0.074	0.388±0.049	0.955	0.515±0.051	0.485±0.018	0.182
	3 300~3 500	0.775±0.163	0.862±0.118	0.497	0.999±0.001	0.996±0.002	0.015

Table 3. Statistical analysis of spectral peak of breast cancer cell lines

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		A549(Mean±SD)		P-value	SPCA(Mean±SD)		P-value
		Control(n=12)	SP(n=12)	P-value	Control(n=12)	SP(n=12)	P-value
Range /cm^-1	1 070~1 090	1 082.166±2.172	1 079.121±2.713	0.021	1 081.871±4.421	1 080.996±5.535	0.468
	1 230~1 250	1 235.728±3.219	1 231.871±3.049	0.018	1 232.353±3.993	1 232.835±4.581	0.516
	1 380~1 420	1 397.004±1.767	1 398.209±3.571	0.329	1 397.004±3.082	1 397.486±3.409	0.626
	1 430~1 470	1 451.245±2.305	1 453.655±5.716	0.38	1 451.486±3.409	1 450.796±1.767	0.782
	1 500~1 600	1 545.021±4.991	1 534.655±5.899	0.001	1 546.468±3.779	1 535.619±3.049	0.001
	1 600~1 700	1 651.092±3.779	1 637.351±8.117	<0.001	1 645.065±4.123	1 638.074±10.254	0.023
	2 910~2 950	2 924.186±1.364	2 924.347±1.575	0.999	2 925.186±1.438	2 925.081±0.941	0.356
	2 950~2 990	2 958.659±0.682	2 959.141±0.998	0.351	2 958.659±0.682	2 958.9±0.893	0.351
	3 300~3 500	3 292.783±2.291	3 293.747±4.305	0.563	3 294.153±2.569	3 293.372±3.404	0.683

Table 4. Spectral peak analysis of lung cancer cell lines

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		A549(Mean±SD)		P-value	SPCA(Mean±SD)		P-value
		Control(n=12)	SP(n=12)	P-value	Control(n=12)	SP(n=12)	P-value
Range /cm^-1	1 070~1 090	0.486±0.17	0.396±0.342	0.075	0.415±0.256	0.297±0.133	0.468
	1 230~1 250	0.463±0.121	0.349±0.283	0.176	0.391±0.102	0.377±0.127	0.505
	1 380~1 420	0.433±0.114	0.346±0.24	0.839	0.388±0.098	0.361±0.129	0.095
	1 430~1 470	0.419±0.115	0.356±0.089	0.57	0.369±0.078	0.375±0.11	0.427
	1 500~1 600	0.687±0.059	0.539±0.209	<0.001	0.656±0.083	0.644±0.153	0.583
	1 600~1 700	0.951±0.018	0.714±0.267	<0.001	0.922±0.077	0.798±0.108	0.018
	2 910~2 950	0.604±0.102	0.524±0.145	0.204	0.491±0.058	0.473±0.039	0.064
	2 950~2 990	0.537±0.088	0.495±0.134	0.342	0.453±0.087	0.465±0.016	0.305
	3 300~3 500	0.993±0.006	0.997±0.003	0.186	0.94±0.056	0.966±0.03	0.358

Table 5. Statistical analysis of spectral peak of lung cancer cell lines

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