Quantum dot materials have the characteristics of narrow luminescence spectrum, adjustable luminescence wavelength and high fluorescence quantum yield. The quantum dot LEDs have more potential in improving color gamut. In this paper, a method of white light generation by blue LED excited CdSe red and green quantum dots is introduced. The ratio of red and green quantum dots to glue was (1∶60) and (1∶10), and the test range of glue content was 1.4 μL～2.2 μL and 3.0 μL～5.0 μL. The samples were prepared by traditional method and layered structure. The absorption and conversion ratio of blue light in the range of glue amount were tested. The function relationship between glue amount and absorption and conversion was obtained by Matlab fitting. When taking the dots (0.34, 0.3) in white light region formed in the above test glue amount range, the red and green quantum dots were calculated with glue amount of 1.9 μL and 4.55 μL, and the corresponding theoretical spectrum was established according to the spectral calculation formula. According to the above glue amount, the verification samples were made and tested the color coordinates (0.3409, 0.2992) and the homologous spectra were basically coincident with the theoretical spectra.