In order to realize the laser cladding remanufacturing and repair of tractor spindle, Ni60A alloy coating was prepared by laser cladding technology on spindle material 40Cr axial surface. The effects of defocusing amount on the macromorphology, microstructure, microhardness, and wear resistance of the cladding layer were analyzed by optical microscope, microhardness tester, and friction wear tester.The results show that when the defocusing amount is 8 mm, the surface of the cladding layer is smooth and flat, and there are no defects such as pores, cracks, and slag inclusion on the bonding surface.In the middle of the cladding layer, there are fine equiaxial crystals, dendrites and columnar crystals on both sides, and planar crystal structures growing along the surface of the substrate at the bottom.The highest hardness was found in the middle of the cladding layer, and its microhardness was up to 660 HV, twice that of the substrate.Under the same wear condition, the wear weight of cladding layer is only 33% of that of matrix material.As the amount of defocusing increases, the surface roughness of cladding layer increases, the hardness non-uniformity increases, and the wear resistance decreases.