• Chinese Journal of Lasers
  • Vol. 46, Issue 12, 1207002 (2019)
Cong Li, Kehong Wang*, and Jun Huang*
Author Affiliations
  • School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, China
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    DOI: 10.3788/CJL201946.1207002 Cite this Article Set citation alerts
    Cong Li, Kehong Wang, Jun Huang. Effects of Indocyanine Green and Methylene Blue on Tensile Strength and Thermal Damage of Laser Biological Tissue Soldering[J]. Chinese Journal of Lasers, 2019, 46(12): 1207002 Copy Citation Text show less
    Experimental instrument and sample. (a) Laser for biological tissue welding and thermal imager; (b) skin sample in vitro and welding position
    Fig. 1. Experimental instrument and sample. (a) Laser for biological tissue welding and thermal imager; (b) skin sample in vitro and welding position
    Test instrument and test processing. (a) Digital explicit push-pull force meter for measuring tensile strength; (b) diagram of process for measuring tensile strength
    Fig. 2. Test instrument and test processing. (a) Digital explicit push-pull force meter for measuring tensile strength; (b) diagram of process for measuring tensile strength
    Macroscopic morphologies of welded specimens of methylene blue test group. (a) Front of welded incision; (b) reverse side of welded incision; (c) side of welded incision
    Fig. 3. Macroscopic morphologies of welded specimens of methylene blue test group. (a) Front of welded incision; (b) reverse side of welded incision; (c) side of welded incision
    Macroscopic morphologies of welded specimens of indocyanine green test group. (a) Front of welded incision; (b) reverse side of welded incision; (c) side of welded incision
    Fig. 4. Macroscopic morphologies of welded specimens of indocyanine green test group. (a) Front of welded incision; (b) reverse side of welded incision; (c) side of welded incision
    Average tensile strength of welded incisions with different biological dyes
    Fig. 5. Average tensile strength of welded incisions with different biological dyes
    Maximum tensile strength of welded incisions with different biological dyes
    Fig. 6. Maximum tensile strength of welded incisions with different biological dyes
    Effects of methylene blue concentration and applied position on tensile strength
    Fig. 7. Effects of methylene blue concentration and applied position on tensile strength
    Effects of indocyanine green concentration and applied position on tensile strength
    Fig. 8. Effects of indocyanine green concentration and applied position on tensile strength
    Average temperature of measuring points of different dyes at different applied positions
    Fig. 9. Average temperature of measuring points of different dyes at different applied positions
    Power /WPulsefrequency /kHzWeldingspeed /(mm·s-1)Weldingtime /s
    41001001200
    Table 1. Process parameters of laser welding for biological tissue
    Type of dyesMass concentration /(mg·L-1)
    SurfaceBack
    5050
    100100
    Indocyanine green150150
    200200
    250250
    5050
    100100
    Methylene blue150150
    200200
    250250
    Table 2. Type, mass concentration, and location distribution of dyes
    Mass concentration of methylene blue /(mg·L-1)Tensile strength /(N·cm-2)
    SurfaceBack
    5014.93±1.228.08±0.89
    10011.10±0.9210.00±0.93
    15011.73±0.789.60±1.30
    20011.20±0.837.80±1.62
    25010.60±1.136.78±0.91
    Table 3. Tensile strength of methylene blue test group
    Mass concentration of indocyanine green /(mg·L-1)Tensile strength /(N·cm-2)
    SurfaceBack
    5011.18±0.808.60±0.91
    10010.88±1.238.90±1.06
    15015.15±1.219.15±1.30
    20013.00±1.3511.90±0.66
    25010.93±0.959.55±0.90
    Table 4. Tensile strength of indocyanine green test group
    AppliedpositionThermal damage
    ControlgroupMethyleneblueIndocyaninegreen
    Surface588174011.7
    Back588193446.9
    Table 5. Thermal damage of measuring points of different dyes at different applied positions
    Cong Li, Kehong Wang, Jun Huang. Effects of Indocyanine Green and Methylene Blue on Tensile Strength and Thermal Damage of Laser Biological Tissue Soldering[J]. Chinese Journal of Lasers, 2019, 46(12): 1207002
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