Author Affiliations
School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, Chinashow less
Fig. 1. Experimental instrument and sample. (a) Laser for biological tissue welding and thermal imager; (b) skin sample in vitro and welding position
Fig. 2. Test instrument and test processing. (a) Digital explicit push-pull force meter for measuring tensile strength; (b) diagram of process for measuring tensile strength
Fig. 3. Macroscopic morphologies of welded specimens of methylene blue test group. (a) Front of welded incision; (b) reverse side of welded incision; (c) side of welded incision
Fig. 4. Macroscopic morphologies of welded specimens of indocyanine green test group. (a) Front of welded incision; (b) reverse side of welded incision; (c) side of welded incision
Fig. 5. Average tensile strength of welded incisions with different biological dyes
Fig. 6. Maximum tensile strength of welded incisions with different biological dyes
Fig. 7. Effects of methylene blue concentration and applied position on tensile strength
Fig. 8. Effects of indocyanine green concentration and applied position on tensile strength
Fig. 9. Average temperature of measuring points of different dyes at different applied positions
Power /W | Pulsefrequency /kHz | Weldingspeed /(mm·s-1) | Weldingtime /s |
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4 | 100 | 100 | 1200 |
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Table 1. Process parameters of laser welding for biological tissue
Type of dyes | Mass concentration /(mg·L-1) |
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Surface | Back |
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| 50 | 50 | | 100 | 100 | Indocyanine green | 150 | 150 | | 200 | 200 | | 250 | 250 | | 50 | 50 | | 100 | 100 | Methylene blue | 150 | 150 | | 200 | 200 | | 250 | 250 |
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Table 2. Type, mass concentration, and location distribution of dyes
Mass concentration of methylene blue /(mg·L-1) | Tensile strength /(N·cm-2) |
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Surface | Back |
---|
50 | 14.93±1.22 | 8.08±0.89 | 100 | 11.10±0.92 | 10.00±0.93 | 150 | 11.73±0.78 | 9.60±1.30 | 200 | 11.20±0.83 | 7.80±1.62 | 250 | 10.60±1.13 | 6.78±0.91 |
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Table 3. Tensile strength of methylene blue test group
Mass concentration of indocyanine green /(mg·L-1) | Tensile strength /(N·cm-2) |
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Surface | Back |
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50 | 11.18±0.80 | 8.60±0.91 | 100 | 10.88±1.23 | 8.90±1.06 | 150 | 15.15±1.21 | 9.15±1.30 | 200 | 13.00±1.35 | 11.90±0.66 | 250 | 10.93±0.95 | 9.55±0.90 |
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Table 4. Tensile strength of indocyanine green test group
Appliedposition | Thermal damage |
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Controlgroup | Methyleneblue | Indocyaninegreen |
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Surface | 5881 | 740 | 11.7 | Back | 5881 | 934 | 46.9 |
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Table 5. Thermal damage of measuring points of different dyes at different applied positions