Intracellular delivery is a critical step in cell engineering and cell therapy. Compared with conventional microinjection, cell fusion, and electroporation, laser-induced intracellular delivery has advantages such as high precision, high throughput, and non-invasiveness. This technology has received increasing attention in recent years. The laser induces cell membrane to produce transient micropores that increase the permeability of the cell membrane and allow the cargo to pass through the cell membrane and go into the cell. This paper mainly introduces the principles that laser uses thermal effect, chemical effect, and fluid shear force to destroy cell membrane. Their implementation methods are divided into direct laser action, and laser action on the nanoparticles, substrate material or photothermal knife to destroy the cell membrane. The advantages and disadvantages of each method are evaluated, and the prospects of the laser-induced intracellular delivery are forecasted.