Manshu Du, Xiaohui Lin, Jiayu Yang, Jianchun Liu, Dongxu Zhang. Calibration Method of Fluorescence Excited Light Path in Nucleic Acid Detection System[J]. Laser & Optoelectronics Progress, 2022, 59(7): 0712003
- Laser & Optoelectronics Progress
- Vol. 59, Issue 7, 0712003 (2022)
Fig. 1. Structure diagram of optical system of nucleic acid detection instrument
Fig. 2. Schematic of excitation light path
Fig. 3. Relation curve between exit angle of light source and radius of light at edge of secondary refraction surface
Fig. 4. Relation curve between exit angle of light source and exit angle of secondary refraction surface
Fig. 5. ZEMAX simulation results
Fig. 6. Experimental setup for measuring light spot
Fig. 7. Images taken by CCD with five different apertures
Fig. 8. Changes of exit spot radius of excitation light path under different apertures
Fig. 9. Images taken by CCD of optical path collimation experiments under different distances between screen and lens. (a) 7.7 mm; (b) 8.7 mm
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Table 1. Parameters table of numerical calculation system for spot radius
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Table 2. Experimental data of collimation of excitation light path
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