The Raman spectra of TiBa glass plate and TiBa glass microsphere were measured and the structural resonance in Raman spectrum o f glass microsphere was found. According to the microcavity theory, the structur al resonance was analyzed, and the Mie scattering theory was used to fit the exp erimental results, showing the investigated microsphere diameter of 25.01μm and the refractive index of 1.895 at a wavelength close to 632.8 nm (with a Raman s hift of 300cm^-1)