The spatial scanning Raman spectra method is applied in the study of fluid inclusion of carbonate reservoir. Some inclusions of carbonate reservoir are exannined by microscopic confocal Raman spectral methods. In experimental process, X-Y scanning and depth profile(Z scanning) have been finished around the inclusion, with sample temperature of 20℃, -40℃, -80℃ and -170 ℃. Because the size of some inclusions are relatively small or hidden deeply in sample, their Raman spectra signals are often buried in some noises and other disturbing signals which stem from main mineral. The experimental results show that the original experimental spectral curve of the inclusion does not almost distinguish from the original experimental spectral curve of pure main mineral(rock core) when relative large size of confocal pinhole is used. It is found that the number aperture (NA) and the fill factor of objective are important to the performance of lateral resolution, whereas the size of confocal pinhole is critical to the longitudinal resolving ability of microscopic confocal Raman spectroscope.