In order to investigate the feasibility of fiber-guided 2.013 μm Q-switched thuliumYAG lasers as one microsurgical scalpel, dissection performance of 200 μm core diameter fiber-guided thuliumYAG lasers operating at 1 kHz repetition rate and 400~1400 ns pulse duration on fresh pig kidney tissues, is researched under different pulse energies and dissection velocities. One professional camera and one optical microscope are used to capture the macro-histological tissue surfaces and the micro-histological craters, respectively. A scientific standard software is used to analyze the experimental data. The experimental results show that the dissection effect is not obvious for higher dissection velocity under the same low energy level. However, it is obvious for higher energy under the same dissection velocity. The fiber-guided high repetition rate Q-switched thuliumYAG lasers will be expected to be used in clinical operation as a microsurgical scalpel.