To improve the fit efficiency of optical membranes and displays and overall alignment precision in autostereoscopic 3D displays, this article proposes an overall, high efficiency and real-time measurement scheme for optical properties of 3D displays. The corresponding measurement system is developed. The real-time measurement of luminance uniformity and crosstalk ratio uniformity for 3D display screen is realized. Photodiode array of the measurement system is designed and developed. Circuit control module with high sensitivity and high precision is fabricated. And software of upper computer is designed to display data in real time. The spectral response of the photodiodes is close to that of human eyes. The calibrated and directionally corrected photodiodes can be used to measure light intensity distributions of visual area from different parts on screen in real time. The crosstalk ratio data from different parts on screen can be calculated quantitatively with obtained data. Experimental results show that the system is capable of effectively measuring spatial light intensity distribution within 23.7 cm width, which covers a pair of eyes′visual area. The measurement precision of the system is 1.58 mlx. Results prove that the data can quantitatively reflect luminance and crosstalk ratio perceived by human eyes. This method can be used to measure light intensity distribution of visual area for 3D displayer in real time, and calculate crosstalk ratio distribution. Which is beneficial to enhance the fabrication efficiency of autostereoscopic 3D display devices.