Infrared conformal seeker has demonstrated its capabilities as a prominent technique for the supersonic characteristic. To achieve the potential accuracy of target tracing, boresight error should be corrected as a result of using aspheric and quadric surfaces in conformal optics system. This paper describes boresight error measurement and correction in conformal infrared seeker. Expression and correlation coefficient of boresight error along with field of regard (FOR) ranging from 0° to 20° are obtained using ray tracing simulation. Boresight error measurement has been accomplished by correlation matching method, and results illuminate that the relative error is 5.02%. Error correction model by unitary nolinear regression analysis is introduced. The experimental results have demonstrated that boresight error is reduced to lower than 30 μm and tracking accuracy is improved to ±1 pixel. The results can meet the precision requirement of servo control system and provide part of the basis for optics system optimization.