In order to analyze the effects of moisture on soil spectra detection, soil samples from three different places (Taoyuanbao, Muchang, and Shanxi agricultural university) were collected. All soil samples were sieved, dried and prepared with different water content (20%, 15%and 10%). The spectra of soil samples with different water content were collected by ASD FieldSpec3. Finally, moisture was looked as the the external disturbance, and two-dimensional correlation near-infrared spectroscopy (2D-NIR) technique was applied to analyze the effect of moisture on soil spectra detection. 2D-NIR analysis was realized by using shige software (2Dshige shigeaki Morita, Kwansei-Gakuin University).The results showed that, the sensitive bands of moisture in 2D-NIR spectra were more obvious than one-dimensional spectra. In the region 350～2 500 nm, the synchronous spectra of soil samples from different places were similar. Two automatic peaks near 2 210 and 1 929 nm were strong, and one automatic peak near 1 415 nm was weak. Moreover, the peak 1 929 nm was most sensitive to moisture content, next was 2 210 nm, and the last one was 1 415 nm. The sensitive bands and sensitive degree were pointed out in this research. It will be helpful for anti moisture model building.