It is necessary to measure and compensate the phase fluctuation introduced by fiber optic links in high-precision fiber frequency transfer. Frequency crosstalk is one of the influence factors, which is easy to be introduced and has a certain impact on the frequency transfer performance. In order to evaluate the impact of frequency crosstalk on fiber frequency transfer performance, the influence model of frequency crosstalk on frequency transfer stability is established. The stability loss caused by frequency crosstalk due to temperature change of fiber link is analyzed by simulation and experiment. The results show that the stability loss of the frequency transfer is related to signal crosstalk factor and the frequency of frequency standard. The larger signal crosstalk factor, the larger the overall up-shift amplitude of Allen deviation curve, and the crosstalk factor is approximately linear with the stability loss peak value. When the frequency of frequency standard changes, the long-term stability of the frequency is not only affected by the slow delay drift caused by the temperature variations, but also related to frequency points and delay fluctuation. If the frequency rises, the peak of the frequency stability loss will move to the short term.