Damage experiments are conducted by irradiating fused silica with the multiple wavelength laser near field. The multiple wavelength laser consists of 1053、 527、 351 nm laser. It designs a definition of damage threshold based on laser near-field irradiation and extracts damage areas from damage images by the marker-based watershed algorithm with gray control. The initial damage threshold is defined as the fluence of critical site between damage region and no damage region, which is calculated by comparing the damage image with the multiple wavelength laser near field. The research shows that the damage of fused silica is induced by the three wavelength lasers. The 351 nm laser plays a leading role. The initial damage threshold is 8.22 J/cm2. With multiple irradiation of fused silica in multiple wavelength laser, the damage growth of exit surface is exponential, and the coefficient of damage growth is 0.59.