The spectrum power of low-pressure mercury is weak, and the spectral lines cannot be traceable to national standard. Using SHG and FHG technique, the laser at 532nm and 266nm was obtained based on 1064nm single solid-state ring laser. Using modulation transfer spectroscopy, the frequency of the 532nm laser was stabilized at 10 hyperfine Spectra of 127I R (56)32-0. The wavelength accuracy of grating spectrograph was calibrated. The experiment results prove that the wavelengths of frequency stabilized wavelength source have strict multiple relationship, and the wavelength accuracy of grating spectrograph can be calibrated accurately and effectively. So this technique has good application value.