Fluorescence Spectrum Characteristics of Fulvic Acid in Black Soil Under Different Ratios of Organic-Inorganic Fertilizers Combined Application

Yan LI; Yang BAI; Dan WEI; Wei WANG; Yu-mei LI; Hong XUE; Yu HU; Shan-shan CAI

doi:10.3964/j.issn.1000-0593(2021)11-3518-06

Journals >Spectroscopy and Spectral Analysis >Volume 41 >Issue 11 >Page 3518 > Article

Spectroscopy and Spectral Analysis
Vol. 41, Issue 11, 3518 (2021)

Fluorescence Spectrum Characteristics of Fulvic Acid in Black Soil Under Different Ratios of Organic-Inorganic Fertilizers Combined Application

Yan LI^1、1;, Yang BAI^1、1;, Dan WEI^{1、1; 2; *;}, Wei WANG^3、3;, Yu-mei LI^3、3;, Hong XUE^4、4;, Yu HU^1、1;, and Shan-shan CAI^5、5;

Author Affiliations

¹1. College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China

³3. Institute of Soil, Fertilizer and Environmental Resources, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

⁴4. Keshan Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161000, China

⁵5. College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China

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DOI: 10.3964/j.issn.1000-0593(2021)11-3518-06 Cite this Article

Yan LI, Yang BAI, Dan WEI, Wei WANG, Yu-mei LI, Hong XUE, Yu HU, Shan-shan CAI. Fluorescence Spectrum Characteristics of Fulvic Acid in Black Soil Under Different Ratios of Organic-Inorganic Fertilizers Combined Application[J]. Spectroscopy and Spectral Analysis, 2021, 41(11): 3518 Copy Citation Text

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Differences of SOC and FA contents in black soil after combined application of organic and inorganic fertilizersNote: Lowercase letters represent significant difference (p<0.05)

Fig. 1. Differences of SOC and FA contents in black soil after combined application of organic and inorganic fertilizers
Note: Lowercase letters represent significant difference (p<0.05)

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Three dimensional fluorescence components of soil FA in different treatmentsPeak A: fulvic-acid-like in UV region; Peak C: fulvic-acid-like in visible region; Peak F: humic-acid-like;Peak T: short wave tryptophan-like (protein-like); Peak T1: long wave tryptophan-like

Fig. 2. Three dimensional fluorescence components of soil FA in different treatments
Peak A: fulvic-acid-like in UV region; Peak C: fulvic-acid-like in visible region; Peak F: humic-acid-like;
Peak T: short wave tryptophan-like (protein-like); Peak T1: long wave tryptophan-like

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Fig. 3. RDA of different treatments, fluorescence intensity and soil carbon content

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土壤层次/cm	pH	有机碳/ (g·kg^-1)	全氮/ (g·kg^-1)	碱解氮/ (mg·kg^-1)	全磷/ (g·kg^-1)	速效磷/ (mg·kg^-1)	全钾/ (g·kg^-1)	速效钾/ (mg·kg^-1)
0~20	6.36±0.02	17.42±1.05	1.81±0.03	129.12±10.59	1.69±0.10	51.26±2.69	18.92±0.34	202.2±21.74

Table 1. Basic chemical properties of soil

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pH	全氮/ (g·kg^-1)	全磷/ (g·kg^-1)	全钾/ (g·kg^-1)	有机碳/ (g·kg^-1)
6.90±0.03	4.68±0.25	2.41±0.12	1.97±0.06	26.62±1.13

Table 2. Basic chemical properties of organic fertilizer

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处理	FI	BIX	HIX
CK	1.63±0.04b	0.70±0.02a	2.04±0.28c
NPK	1.65±0.04ab	0.72±0.03a	2.36±0.16b
NPKM1	1.67±0.02ab	0.74±0.02a	2.51±0.29ab
NPKM2	1.71±0.03a	0.75±0.05a	2.94±0.41a
NPKM3	1.67±0.06ab	0.74±0.06a	2.58±0.31ab
NPKM4	1.70±0.03ab	0.75±0.04a	2.78±0.51a

Table 3. Fluorescence spectrum indices of soil FA

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处理	C1 (R.U.)	C2 (R.U.)	C3 (R.U.)	C1+C2+C3	相对百分比/%
处理	C1 (R.U.)	C2 (R.U.)	C3 (R.U.)	C1+C2+C3	C1	C2	C3
CK	1 656.87±181.18c	685.59±91.36c	527.13±59.03b	2 869.58±331.57c	57.74	23.89	18.37
NPK	2 082.61±37.51ab	874.87±79.68ab	573.75±50.47ab	3 531.23±167.66a	58.98	24.78	16.25
NPKM1	2 060.98±105.37ab	831.19±69.77b	603.27±82.66a	3 495.44±257.80a	58.96	23.78	17.26
NPKM2	2 127.42±124.08a	906.28±94.36a	535.28±283.05b	3 568.98±167.03a	59.61	25.39	15.00
NPKM3	1 884.82±105.92bc	704.97±199.71c	535.42±109.46b	3 125.21±415.09b	60.31	22.56	17.13
NPKM4	1 779.44±180.66c	727.67±167.62bc	490.93±24.85c	2 998.05±373.13b	59.35	24.27	16.38

Table 4. Fluorescence intensity and relative percentage of soil FA fluorescence components

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