The spectroscopic parameters of gas absorption lines are an important factor that can influence gas parameters’ measurement accuracy based on laser absorption spectroscopy technology. However, the spectroscopic parameters in the molecule spectral database exist big uncertainties, resulting in the obvious measurement errors when used in laser absorption technology measurement. To obtain the spectroscopic parameters of H2O lines that can be used in the combustion diagnosis, time-division multiplexing technique is adopted to measure the absorption spectroscopy near 1.4 μm in the environments where the temperature, pressure and H2O concentration are known. The line strength, broadening coefficients and their temperature-dependent exponents of two H2O lines (7 185.60 and 7 454.45 cm-1) are measured, and the measured line strength of the two lines is less than 2.61% and 4.65% compared to the value in the HITRAN database. The uncertainty of the measured line strength of two lines is less than 4%.