Based on the birefringent characteristic of thin film fabricated by glancing angle deposition(GLAD),a birefringent non-polarizing thin film at wavelength 632 nm only using titanium oxide is designed and deposited. At the beginning,two titanium oxide monolayers were deposited with the deposition angle 60° and 70° respectively. The effective refractive index of the monolayer for s- and p-polarization,nPH,nSH and nPL,nSL ,were obtained. a higher s transmittance than p polarization at normal incidence is got by arranging the index correctly,since the increase of the incidence would bring an opposite effect,we could get a non-polarizing effect at a very angle can be got. Based on the analysis above,a non-polarizing thin film was designed and prepared. The s- and p-polarization transmission spectra of non-polarizing film were measured with photometer at normal and tilted incidence. At the incidence angle of 60°,the reflection band width of s- and p-polarizations were less different and the non-polarizing effect was achieved partially. The obliquely incident non-polarizing thin film can be designed based on the thin film material′s birefringence.