Abstract
Introduction
Structured light illumination is a special illumination method which is effective in performing 3D shape measurement and machine vision.[
In this letter, a novel portable and economical structured light generation system based on an optoelectronic integrated chip with a broad bandwidth is proposed. In this method, lights coupled out from specific gratings interfere mutually above the chip and afterwards generate the necessary excitation patterns of structured illumination microscopy (SIM). An upright microscope is placed directly on the top of the integrated chip to gather specimen images. The beam intensities and phases steps are modulated on the chip taking advantage of thermo-optic or electro-optic effect. This design enables beam control without the need for free-space bulky equipment. The integrated chip is based on silicon-on-insulator(SOI), which has been mentioned in our previous work.[
1 Chip design
The layout of the structured illumination chip composes with three crucial parts, which are input and output grating couplers, beam splitters and intensity and phase step modulators. The schematic of the on-chip optics is shown in
Figure 1.The sketch of on-chip optics. GC is grating coupler; BPS is beam power splitter and BM is beam modulator including intensity and phase modulator.
1.1 Devices on the chip
1.1.1 Splitters
To achieve applicable structured light, two or more light beams need to interfere with each other. It is crucial to split the light evenly to ensure high fringe contrast and obtain a high quality interference light pattern. A tradition method to split beam is using directional coupler(DC), which is wavelength sensitive and requires high fabrication accuracy. Two other suitable ways to split the beam on-chip are investigated.
Figure 2.The Schematic diagram of splitters (a)1*2 and 1*3 MMI and(b) 1*2 and 1*3 ADC
1.1.2 Beam modulators
After splitting the light, the phase and amplitude of each beam need to be modulated. The principle of phase shifters is to control the refractive index in the SOI waveguides by means of either electro-optic or thermo-optic effect. By designing the electrodes reasonably and controlling the voltages applied to them, the desired phase can be obtained. The intensities of the beams are modulated using Mach-Zehnder interferometer (MZI) structures with two arms containing phase shifters. The light intensity is determined by the phase difference between beams from two arms. When the output light passes through the way of constructive interference, the intensity of light is maximum; when the light passes through the way of destructive interference, the intensity of light is minimum. The MZI structure with a 1:1 optical splitter can not only control the intensity of the light but also determine the way of light propagation. So it can also be used as an optical switch. The on-chip modulation method allows low transmission loss, low cost, high stability and low power consumption.
1.1.3 Gratings
Since the interference occures above the chip, another key component is the input and output gratings. Due to the difference in effective refractive index, transverse-magnetic (TM) polarized mode cannot be efficiently coupled through the grating couplers. Only transverse-electric (TE) mode is considered in the design. Considering that the light beam is injected from the fiber to the input side of the waveguide through the grating coupler, a Gaussian-like beam which can match the field profile emerging from fiber is wanted. That ensures higher coupling efficiency and lower input loss. The far field beam intensity distribution coupled out from the gratings are expected to be approximate planar light. Taking these factors into consideration, a non-uniform grating coupler is designed. The grating periods, fill factors and etch depths are carefully designed using a formula method. More details can be found in our previous work [
1.2 Calculation and simulation of interference patterns
The generation of interference pattern requires at least two inverse gratings. In most cases, a structured light pattern is generated by the light coupled out from two gratings with a direction of 180 degrees or three gratings with a direction of 120 degrees. Taking two opposite gratings as an example, the density of interference fringes is related to the distance between the two gratings and the output coupling angle of the two beams. Ideally, we can model the interference as Young's Double-slit Interference. The interference period can be easily calculated by the equation (1).
where is the center wavelength of output light, d is the height of the interference plane, L is the distance between two gratings’ center,is the angle between the output beam and the chip surface, as
Figure 3.The FDTD simulation of interference pattern of two beams at the wavelength of 1550 nm. The distance between the two grating is about 200 μm (a) The sketch of GC structure where θ is the output angle,(b) Intensity distribution of beam coupled out from GC,(c) The sketch of interfering process,(d) The distribution of light intensity above the chip,(e) and (f) The distribution of light intensity at the height of 400 and 500 μm upon the chip
In practice, the beams coupled out from gratings cannot be regarded as ones ejected from point sources. FDTD simulation is taken to research what the interference pattern looks like. Taking the distance between two gratings 200 μm as an instance, the result shows that the best interference pattern appears at the height of 400 to 500 μm above the chip. Using this simulation, the period of the interference pattern of each height can also be calculated.
2 Experimental setup
In the experiment, the integrated chip is fabricated on a standard SOI wafer with a 340 nm top silicon thickness and a 1µm buried oxide layer. Electron beam lithography (EBL) and inductively coupled plasma (ICP) etching are used to define the waveguides and gratings by 210 nm etching depth considering the coupling efficiency of the grating couplers. The waveguide widths are designed to be 400 to 600 nm to transmit fundamental mode beam. The heating metals are Ti and Au with thicknesses of 100 nm and 50 nm. Al foil is used as electrode and square pads are designed to connect external circuit. In order to effectively modulate the intensities and phases of output lights through the opto-thermal effect, long and narrow heating metals are used. According to the design of our chip, the width of heating metal is 10 μm and the length is 1100 to 1500 μm. The fabricated chip is pasted on a Printed Circuit Board(PCB) and the electrical pads are connected with external circuits through gold wires. Therefore, the voltage on the heater can be controlled by modulating the external circuit.
A fiber with a 40°inclined surface is horizontally packaged upon the input grating by UV curing adhesive. The single input design ensures uniformity of light polarization and intensity. A tunable laser (AQ2200-136, Yokogawa) is used as light source in this experiment and the wavelength of 1550 nm is taken into use as a representative. After coupled into the waveguide, the light will be separated and transmit through multiple waveguides. The intensity and phase of each beam will be independently modulated. Finally, the light will be coupled out through particular output gratings and interfere with each other upon the chip.
Figure 4.(a) The microscope photograph of the integrated chip on PCB. Gold wires link the chip circuit and the external circuit,(b) Inclined surface fiber is packaged to the chip using UV adhesives,(c) and (d) Scanning electron microscope (SEM) photographs of the integrated chip
The imaging system consists of a CCD camera, a lens column, several lenses and groups of multi-axis translation stages. The composed chip is stuck on a micrometer xyz-stage whose height can be controlled precisely by a computer. The microscope is suspended from the chip via a xyz-translation holder. The objective lens used in this experiment is the Mitutoyo 50× lens of with an N.A. of 0.42. Images are acquired using an infrared camera (HAMAMATSU C10633-23 CMOS camera) linking to the column by a C-mount adapter.
Figure 5.The experiment setup used for SIM imaging. (a) The stretch of the experiment setup; (b) The experiment setup on the optical platform.
3 Experiments and results
Figure 6.Photographs from the CCD camera show the interference patterns at different positions.(a) and (c) Beam spot above the chip along two orthogonal directions,(b) and (d) Interference patterns of two orthogonal directions at the height of 500μm above the chip,(e) Three images with same output gratings but different phase steps
4 Conclusion
In conclusion, a novel compact photonic integrated chip is proposed to generate structured light patterns. This is the first time using only on-chip devices to generate and control infrared structured light. Compared with traditional methods, this method not only is flexible and economical but also has large tolerance of external disturbance and broad bandwidth. Although infrared structured illumination cannot be used in super-resolution imaging, it can be widely applied in 3D shape imaging of biomedical field owing to its absorption character of infrared beam for water. If super-resolution imaging is wanted, other waveguide materials which can transmit visible light such as Si3N4 or Ta2O5 can be applied in this design. It is also possible to expand utilizations of the on-chip modulation methods in other research by developing new illumination patterns in the future.
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