Fig. 1. Schematic of digital holographic microscopy tomography optical path

Fig. 2. Phase images of SiO₂ microsphere at different angles. (a) 90°；(b) 180°；(c) 0°

Fig. 3. Three-dimensional tomographic reconstruction results of SiO₂ microsphere. (a) Central slice in x-y plane; (b) central slice in x-z plane; (c) central slice in y-z plane; (d) three-dimensional tomographic reconstruction result of SiO₂ microsphere

Fig. 4. Holograms at different angles and at the same position and their corresponding spectra, where the area inside the circle is selected filtering window. (a)(d) 90°；(b)(e) 180°；(c)(f) 0°

Fig. 5. Variation of focusing criterion with focusing distance and corresponding amplitude images of different focusing distances, where rectangle represents the selected focus window. (a) Variation of focusing criterion with focusing distance, focusing distance corresponding to the lowest point of the curve is the best reconstruction distance; (b)－(d) reconstruction amplitude images, focusing distance is －3.00, －1.51, and 0 cm, respectively

Fig. 6. Cell phase images before and after phase unwrapping. (a) Cell phase image before phase unwrapping; (b) cell phase image after unwrapping

Fig. 7. Phase images of bladder cancer cells with vacuoles, phase distribution along the dotted line of phase images, and three-dimensional display results of phase images. (a) Cell 1; (b) cell 2; (c) cell 3; (d) cell 4

Fig. 8. Three-dimensional tomographic reconstruction results of bladder cancer cells with vacuoles. (a) Cell 1; (b) cell 2；(c) cell 3; (d) cell 4

Table 1. Three-dimensional morphological parameters of each cell