Lipid droplets imaging with three-photon microscopy

Mubin He; Hojeong Park; Guangle Niu; Qiming Xia; Hequn Zhang; Ben Zhong Tang; Jun Qian

doi:10.1142/S179354582250033X

Journals >Journal of Innovative Optical Health Sciences >Volume 16 >Issue 4 >Page 2250033 > Article

Journal of Innovative Optical Health Sciences
Vol. 16, Issue 4, 2250033 (2023)

Lipid droplets imaging with three-photon microscopy

Mubin He^1、†, Hojeong Park^2、†, Guangle Niu³, Qiming Xia⁴, Hequn Zhang¹, Ben Zhong Tang^5、*, and Jun Qian^1、**

Author Affiliations

¹State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou 310058, P. R. China

²Department of Chemistry, The Hong Kong Branch of Chinese, National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, P. R. China

³Shenzhen Research Institute of Shandong University, Shenzhen 518057, P. R. China

⁴Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, P. R. China

⁵School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, P. R. China

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DOI: 10.1142/S179354582250033X Cite this Article

Mubin He, Hojeong Park, Guangle Niu, Qiming Xia, Hequn Zhang, Ben Zhong Tang, Jun Qian. Lipid droplets imaging with three-photon microscopy[J]. Journal of Innovative Optical Health Sciences, 2023, 16(4): 2250033 Copy Citation Text

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Abstract

Lipid droplets (LDs) participate in many physiological processes, the abnormality of which will cause chronic diseases and pathologies such as diabetes and obesity. It is crucial to monitor the distribution of LDs at high spatial resolution and large depth. Herein, we carried three-photon imaging of LDs in fat liver. Owing to the large three-photon absorption cross-section of the luminogen named NAP-CF₃ (

1.67 \times 1 0^{- 79}

cm⁶ s²), three-photon fluorescence fat liver imaging reached the largest depth of 80

μ

m. Fat liver diagnosis was successfully carried out with excellent performance, providing great potential for LDs-associated pathologies research.

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