The linear absorption, single-photon fluorescence and three-photon absorption properties of two novel fluorene-based derivatives (4-(7-(4-(dimethylamino) phenyl)-9, 9-bis (2-ethylhexyl)-9H-fluorenee-2-yl)-N, N-dimethylbenzenamine (abbreviated as B(DMAP)F) and 4-(2-(7-(4-aminostyryl)-9, 9-bis(2-ethylhexyl)-9H-fluoren-2-yl) vinyl) benzenamino (abbreviated as BASF) with D-π-D structural motifs were reported in THF and DMF solvents. The results show that the absorption peaks and emission peaks of two compounds all have red shift induced by solvent effect. It was found that the three-photon absorption cross-section becomes larger in the more polarity solvent and maximal cross-section is in the order of 10-76 cm6 s2. Large optical power limiting induced by three-photon absorption has also been demonstrated for both dyes. When the incident irradiance reaches 8 GW/cm2, the limiting nonlinear transmissions of B(DMAP)F and BASF can reach 35% and 39%, respectively.