In order to evaluate the anti-jamming performance of the medium and long wave infrared dual-color detecting system, the dual-color target and interference simulation technology were studied. Based on multi-band infrared targets and interference generation technology, the multi-band target and jamming spectral radiation model and motion model were established. At the same time, based on the medium and long wave dual-color hardware-in-the-loop simulation system, through the medium and long wave infrared image simulator composed of MOS resistance array and DMD target simulator, multi-channel compound directional optical system and missile target attitude simulation system with the detection system under test, the medium and long dual-color image generated by the target simulator was compounded, collimated and processed by multi-channel compound directional optical system. After beam expansion, it is provided to the dual-color detection system to verify the simulation effect.