During the weathering and migration of sea surface oil, different oil spill emulsions will be formed, which will cause great harm to the marine environment. Scientific quantification of oil spilt emulsions is helpful for oil spill emergency treatment and disaster damage assessment. Due to the lack of systematic experimental data, physical and chemical and optical parameters, the fine spectral response characteristics and variation rules of different types of oil-water emulsions are not clear, and the data relationship between the spectra of different types of oil spilt emulsions, and the surface oil-water ratio of seawater can not be given. In this paper, through the laboratory experiment of light oil emulsion, using laser-induced fluorescence technology, starting with the difference and change law of fluorescence spectrum response of different types and different surface oil-water ratio, the relevant data of emulsified diesel oil is used as the modeling sample, and the relevant data of emulsified kerosene as the verification sample, the statistical analysis is carried out, and the prediction models of surface oil-water ratio under two types of water in oil and oil in water were designed respectively. In the process of data processing, in order to eliminate the influence of the LIF system on the intensity of the fluorescence signal received, the Raman scattering signal of water is used to normalize the fluorescence signal of emulsion and the ratio of the two is used as the subsequent analysis data. The specific data research shows that the non-linear regression model can be established between the logarithm of fluorescence peak value and the logarithm of the surface water content of oil in oil emulsion spiltoil; the non-linear regression model can also be established between the fluorescence peak value and surface water content of oil in water emulsion oil spill. The correlation coefficients of non-linear fitting were above zero points nine.That is, the model has high quality. The coefficients in the model depend on different oil types and different characteristic fluorescence peaks.It can be seen that the fluorescence peaks of different emulsified oils have the same change trend with the surface oil-water ratio, but the degree of change is different. On this basis, a parameter look-up table is used to estimate the oil-water ratio of light oil emulsions. The surface oil-water ratio can be inverted according to the fluorescence relative intensity.To a certain extent, this method can effectively quantify the oil emulsion on the sea surface, and provide a theoretical basis and basis for the real-time and accurate quantitative analysis of oil spill emulsion in the future, and also provide technical reference for the emergency treatment of oil spill pollution on the sea surface, so it has important research significance and practical value.