Na lidar, meteor radar and medium frequency (MF) radar are three significant techniques in measuring mesospheric horizontal wind fields and were extensively used nowadays. With the three instruments, simultaneous observations were operated for many nights in Hawaii from 2002 to 2005. The horizontal winds observations as long as 8 hours at two nights of July 9, 2002 and October 23, 2003 were chosen and analyzed to study the differences of the three techniques. It is found that the wind amplitudes observed by Na lidar and meteor radar are agreeable with each other normally, but both much larger than MF radar observations. The vertical variations of the nightly mean wind observations show that the three instruments could obtain consistent (evidently discrepant) wind observations when the wind amplitudes and the corresponding shear are weak (strong).