• NUCLEAR TECHNIQUES
  • Vol. 48, Issue 3, 030302 (2025)
Shengnan REN, Linlin LI, Yun ZHANG, Yaling ZHOU..., Tao JIANG, Kejia GAO* and Fang FENG*|Show fewer author(s)
Author Affiliations
  • Department of Nuclear Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai 200434, China
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    DOI: 10.11889/j.0253-3219.2025.hjs.48.240124 Cite this Article
    Shengnan REN, Linlin LI, Yun ZHANG, Yaling ZHOU, Tao JIANG, Kejia GAO, Fang FENG. Preclinical evaluation of osteogenic effects of 99Tc-MDP in osteoporosis models[J]. NUCLEAR TECHNIQUES, 2025, 48(3): 030302 Copy Citation Text show less
    Dynamic changes of blood indexes and the protocol of 99Tc-MDP treatment of osteoporosis model(a) Blood calcium, (b) Blood phosphorus, (c) CTX-I, (d) P1NP, (e) Protocol of 99Tc-MDP treatment
    Fig. 1. Dynamic changes of blood indexes and the protocol of 99Tc-MDP treatment of osteoporosis model(a) Blood calcium, (b) Blood phosphorus, (c) CTX-I, (d) P1NP, (e) Protocol of 99Tc-MDP treatment
    Variation of bone density illustrated in imaging (a~d) For SPECT/CT at 4 weeks, (e) 99Tcm-MDP uptake of knee joint was quantified, (f~i) For SPECT/HRCT at 8 weeks, 99Tcm-MDP uptake (j) and CT values (k) of knee joint were quantified, where A is negative control group, B is model control group, C is 99Tc-MDP treatment group, D is zoledronic acid treatment group
    Fig. 2. Variation of bone density illustrated in imaging (a~d) For SPECT/CT at 4 weeks, (e) 99Tcm-MDP uptake of knee joint was quantified, (f~i) For SPECT/HRCT at 8 weeks, 99Tcm-MDP uptake (j) and CT values (k) of knee joint were quantified, where A is negative control group, B is model control group, C is 99Tc-MDP treatment group, D is zoledronic acid treatment group
    Changes of blood calcium level (a) and blood phosphorus content (b) during the treatment of osteoporosis (color online) where A is negative control group, B is model control group, C is 99Tc-MDP treatment group, D is zoledronic acid treatment group
    Fig. 3. Changes of blood calcium level (a) and blood phosphorus content (b) during the treatment of osteoporosis (color online) where A is negative control group, B is model control group, C is 99Tc-MDP treatment group, D is zoledronic acid treatment group
    Diagrams of imbalance and re-balance of OB/OC (color online). The quantification of distance between green and red fluorescence (a), area of stained OB (b) and intensity of stained OB (c), and typical fluorescence, IHC staining of ALP and TRAP, and HE staining were summarized in (d), where A is negative control group, B is model control group, C is 99Tc-MDP treatment group, D is zoledronic acid treatment group
    Fig. 4. Diagrams of imbalance and re-balance of OB/OC (color online). The quantification of distance between green and red fluorescence (a), area of stained OB (b) and intensity of stained OB (c), and typical fluorescence, IHC staining of ALP and TRAP, and HE staining were summarized in (d), where A is negative control group, B is model control group, C is 99Tc-MDP treatment group, D is zoledronic acid treatment group
    项目 ItemGroup AGroup BGroup CGroup D
    IFN-γ17.039±1.77223.888±3.52525.261±3.11015.653±2.905
    IL-1057.428±7.90570.266±10.55475.238±5.95560.763±10.126
    IL-135.599±0.7527.554±2.44710.393±0.9534.859±0.902
    IL-1β12.473±3.5593.318±1.337NaN1.502±0.552
    IL-43.628±1.3074.824±0.9825.042±0.7793.598±1.053
    IL-5NaNNaNNaN5.545±1.557
    IL-615.927±5.31030.777±7.09843.026±5.11719.200±5.205
    KC/GRO79.765±8.835484.108±29.926318.540±39.558169.990±17.737
    TNF-α35.661±5.1152.322±1.0272.785±0.7472.221±0.557
    Table 1. Multi-cytokine analysis
    Shengnan REN, Linlin LI, Yun ZHANG, Yaling ZHOU, Tao JIANG, Kejia GAO, Fang FENG. Preclinical evaluation of osteogenic effects of 99Tc-MDP in osteoporosis models[J]. NUCLEAR TECHNIQUES, 2025, 48(3): 030302
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