• Spectroscopy and Spectral Analysis
  • Vol. 41, Issue 2, 540 (2021)
Xing-hu FU*, Fei ZHAO, Zhen-xing WANG, Xin LU, Guang-wei FU, Wa JIN, and Wei-hong BI
Author Affiliations
  • School of Information Science and Engineering, The Key Laboratory for Special Fiber and Fiber Sensor of Hebei Province, Yanshan University, Qinhuangdao 066004, China
  • show less
    DOI: 10.3964/j.issn.1000-0593(2021)02-0540-06 Cite this Article
    Xing-hu FU, Fei ZHAO, Zhen-xing WANG, Xin LU, Guang-wei FU, Wa JIN, Wei-hong BI. Quantitative Analysis of Goat Serum Protein Content by Raman Spectroscopy Based on IABC-SVR[J]. Spectroscopy and Spectral Analysis, 2021, 41(2): 540 Copy Citation Text show less
    Raman processing(a): 26 groups of original Raman spectra; (b): Smoothing processing; (c): Background baseline deduction; (d): Normalization
    Fig. 1. Raman processing
    (a): 26 groups of original Raman spectra; (b): Smoothing processing; (c): Background baseline deduction; (d): Normalization
    Characteristic peak of Raman spectrum of serum protein
    Fig. 2. Characteristic peak of Raman spectrum of serum protein
    Modeling process flow chart
    Fig. 3. Modeling process flow chart
    Correlation curve between actual value and predicted value in model test set
    Fig. 4. Correlation curve between actual value and predicted value in model test set
    Residual of model prediction
    Fig. 5. Residual of model prediction
    Correlation curve between actual value and predicted value in three models’ prediction results
    Fig. 6. Correlation curve between actual value and predicted value in three models’ prediction results
    拉曼位移
    /cm-1
    振动模式初步指认主要归属
    379.05Trp色氨酸
    451.87ManD-甘露醇
    568.07ν(s-s)二硫键伸缩振动
    636.12ν(s-s), Tyr酪氨酸
    709.73γ(c-s)辅酶
    797.08Asn天冬酰胺
    864.21Trp色氨酸
    920.15ν(c-c), ManD-甘露醇
    1 009.95ν(c-n), Man, Amide-ⅢD-甘露醇, 碳氮键伸缩振动, 酰胺-Ⅲ
    1 072.24Beta carotene, Tyrβ-胡萝卜素, 酪氨酸
    Table 1. Characteristic peak of serum protein and its attribution
    MSErTime/s
    BP0.681 20.971 4623.5
    ABC-SVR0.321 20.979 472.5
    IABC-SVR0.244 30.990 271.9
    Table 2. Evaluation results of three modeling methods
    Xing-hu FU, Fei ZHAO, Zhen-xing WANG, Xin LU, Guang-wei FU, Wa JIN, Wei-hong BI. Quantitative Analysis of Goat Serum Protein Content by Raman Spectroscopy Based on IABC-SVR[J]. Spectroscopy and Spectral Analysis, 2021, 41(2): 540
    Download Citation