To study the effects of the pyrimidine ring with -NH2 on transient luminescence properties in ruthenium complexes bonding to DNA, transient luminescence dynamics process of two ruthenium complexes [Ru(bpy)2(dpbpd(NH2)2)]2+ and [Ru(phen)2(dpbpd(NH2)2)]2+bonding to calf thymus DNA(ctDNA) have been studied by using time-resolved spectroscopy, and the results are compared with results in previous researches. Two complexes both show biexponential decay in emission on bonding to DNA, and the luminescence lifetime is within tens of nanoseconds, about ten times shorter than the luminescence lifetime (several hundreds nanoseconds) of dppz-kind ruthenium complexes. It is attributed to that the hydrogen-bonds may form between N or -NH2 of pyrimidine ring and water molecule, base pairs or phosphoric skeleton. The hydrogen-bonds can quicken the nonradiative decay of the excitated state, weaken the luminescence intensity, and shorten the luminescence lifetime. The conclusion offers an argument to study the interaction mechanism for the complex bonding to DNA.